Spectrum Mill - MRM Selector
Spectrum Mill
Spectrum Summary
Help
Select Results for MRM Selection
Queue request
Format:
MassHunter QQQ DMRM
MassHunter QQQ MRM
MassHunter QQQ Optimizer
ABI Triple Quad
Filter to distinct peptides:
Off
Case insensitive
Case sensitive
Charge file CS
Data directories:
(None Selected)
Search result files:
*.spo
Validation and Sorting
Filter results by:
sequence-not-validated
valid
good-spectrum-sequence-not-validated
all
Protein grouping method:
1 shared peptide
1 shared, expand subgroups
-
Sort proteins by:
Score
Number of Peptides
Protein MW
Category
Function
Filter by protein score:
>
<
Top
n
peptides for MRM:
Limit to
Take All
Rank peptides by:
Score
Total intensity
Sort MRM List by:
Precursor m/z
Precursor MH+
Score
Sequence
Sequence file charge
Accession number
Total intensity
Retention Time
Filter peptides by:
Score:
>
<
% SPI:
>
<
Required AAs: Disallowed AAs:
Peptide pI: from
to
All
Accession #'s:
MRM Parameters
Text file export
Simple sequence
Top
n
Transitions:
≥
% of precursor
y
-ions only
Trigger threshold:
for
secondary transitions
Z options:
Observed precursor/fragment charge only
Exhaustive precursor and fragment charges
Highest allowed charge pre/frags only
Dwell time (ms):
Use peak width of:
min
Collision Energy:
(CE= slope*m/z + intercept)
Use actual CE if available
z-state:
slope:
intercept:
2
3
4
Declustering Potential:
m/z breakpoint:
Potential:
Less than
in between
More than
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