Agilent Spectrum Mill MS Proteomics Workbench

Mutation Mass Shifts

This table lists the delta masses between various amino acids and can be used to help explain how an observed mass change could represent an AA mutation.

Residues DOWN the left indicate the EXPECTED residues.

Residues ACROSS the top indicate the MUTANT residues.

		G	A	S	P	V	T	C	L/I	N	D	Q/K	E	M	H	F	R	Y	W
		57	71	87	97	99	101	103	113	114	115	128	129	131	137	147	156	163	186
		________________________________________________________________________
G	57		14	30	40	42	44	46	56	57	58	71	72	74	80	90	99	106	129
A	71	-14		16	26	28	30	32	42	43	44	57	58	60	66	76	85	92	115
S	87	-30	-16		10	12	14	16	26	27	28	41	42	44	50	60	69	76	99
P	97	-40	-26	-10		2	4	6	16	17	18	31	32	34	40	50	59	66	89
V	99	-42	-28	-12	-2		2	4	14	15	16	29	30	32	38	48	57	64	87
T	101	-44	-30	-14	-4	-2		2	12	13	14	27	28	30	36	46	55	62	85
C	103	-46	-32	-16	-6	-4	-2		10	11	12	25	26	28	34	44	53	60	83
L/I	113	-56	-42	-26	-16	-14	-12	-10		1	2	15	16	18	24	34	43	50	73
N	114	-57	-43	-27	-17	-15	-13	-11	-1		1	14	15	17	23	33	42	49	72
D	115	-58	-44	-28	-18	-16	-14	-12	-2	-1		13	14	16	22	32	41	48	71
Q/K	128	-71	-57	-41	-31	-29	-27	-25	-15	-14	-13		1	3	9	19	28	35	58
E	129	-72	-58	-42	-32	-30	-28	-26	-16	-15	-14	-1		2	8	18	27	34	57
M	131	-74	-60	-44	-34	-32	-30	-28	-18	-17	-16	-3	-2		6	16	25	32	55
H	137	-80	-66	-50	-40	-38	-36	-34	-24	-23	-22	-9	-8	-6		10	19	26	49
F	147	-90	-76	-60	-50	-48	-46	-44	-34	-33	-32	-19	-18	-16	-10		9	16	39
R	156	-99	-85	-69	-59	-57	-55	-53	-43	-42	-41	-28	-27	-25	-19	-9		7	30
Y	163	-106	-92	-76	-66	-64	-62	-60	-50	-49	-48	-35	-34	-32	-26	-16	-7		23
W	186	-129	-115	-99	-89	-87	-85	-83	-73	-72	-71	-58	-57	-55	-49	-39	-30	-23

All values are (column-row). The mass difference observed is consistent with a ROW to COLUMN mutation.