Spectrum Mill - MS Product
Spectrum Mill
MS Digest
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Fragmentation
Calculate masses as:
Vertical format saved to file
Maximum reported charge:
No Limit
1
2
3
4
5
Peptide Sequence
Use
CAPITAL
letters for unmodified amino acids or those with Fixed Modifications.
Use
lower case
letters for amino acids to indicate a Variable Modification (eg. KCmqYQ).
Use 'u' for the user-specified aminio acid (see below).
Use 'o' for Homoserine Lactone.
Enter sequence:
Modifications
Fixed:
Variable:
Product Ion Types
(some types require presence of specific AA's in ion)
AA composition ions
N-terminus sequence ions
C-terminus sequence ions
Internal
product ions
Ladder
sequencing ions
i
m
a
b
c
c'
x
y
Y
z
z'
z''
internal
N-terminus
C-terminus
Satellite sequence ions
( side-chain loss )
Neutral-loss sequence ions
Peeling sequence ions
d
v
w
-H
2
O
S, T
-NH
3
R, K, Q
-H
3
PO
4
s, t
( S, T + PO
4
)
-SOCH
4
m
( M + Ox)
b+H
2
O
R, H, K
User-specified AA elemental composition (u):
C:
H:
N:
O:
S:
P:
( Default is Gly )